GALLERY

PUBLICATIONS/PREPRINTS

Jeong, Kim, et al. (2016)  Comparative three-dimensional connectome map of motor cortical projections in the mouse brain. Scientific Reports 6(1):20072. [Link]

ABOUT

The goal is to generate brainwide maps of cell-type specific long-range connectivity, establishing a quantitative approach to the study of cell-type distribution and connectivity in the mouse brain. These maps will thus specify the inputs and outputs of neuronal and glia populations in every brain region, corresponding to brain compartments defined in classical neuroanatomy.

To determine the cell-types of a brain region, reporter mice expressing Cre (or Flp)recombinase “tag” from cell-specific marker genes are used. The current list of such reporter mice includes most of the major known cell types in the brain and additional mice for more specialized subtypes are being generated based on advanced transcriptional profiling and the use of intersectional strategies combining two cell-marker genes. In addition, anatomical connections of the tagged cell populations can be mapped by recombination-dependent vectors for anatomical tracing, including transsynaptic labeling. In summary, it is now possible to study the function and anatomy of cell types in the mouse brain at an unprecedented spatial and temporal resolution. The whole brain is then sliced thinly, and each slice is digitally imaged. These 2-D images are reconstructed in 3D. This procedure is repeated identically, to account for individual variability.

This project provides the means to measure the numbers and ratios of specific cell types per brain areas during the critical time when neuronal circuits come together to initiate brain functions. This work will also reveal the extent to which programmed cell death of specific types is part of circuit wiring and pruning in specific brain areas. In order to accumulate data from different mice (each of whom has a slightly different brain shape and size), 3-D spatial normalization is performed using registration algorithms.

DATASETS

desc Metadata Image Viewer
MouseBrain_OST1 F Region:Primary motor area; Tracer:AAV2/1.CAG.tdTomato.WPRE.SV40
MouseBrain_OST3 F Region:Primary motor area; Tracer:AAV2/1.CAG.tdTomato.WPRE.SV40
MouseBrain_OST5 F Region:Primary motor area; Tracer:AAV2/1.CAG.tdTomato.WPRE.SV40
MouseBrain_OST6 F Region:Primary motor area; Tracer:AAV2/1.CAG.tdTomato.WPRE.SV40
MouseBrain_OST7 F Region:Primary motor area; Tracer:AAV2/1.CAG.tdTomato.WPRE.SV40
MouseBrain_OST8 F Region:Primary motor area; Tracer:AAV2/1.CAG.tdTomato.WPRE.SV40
MouseBrain_OST9 F Region:Primary motor area; Tracer:AAV2/1.CAG.tdTomato.WPRE.SV40
MouseBrain_OST10 F Region:Primary motor area; Tracer:AAV2/1.CAG.tdTomato.WPRE.SV40
MouseBrain_OST11 F Region:Primary motor area; Tracer:AAV2/1.CAG.tdTomato.WPRE.SV40
MouseBrain_OST4 F Region:Primary motor area; Tracer:AAV2/1.CAG.tdTomato.WPRE.SV40
MouseBrain_HUA_U19_180313 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180402 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_170519 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_171005 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180514 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_170928 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181115 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180816 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181015 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180515 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180722 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180809 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181011 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180725 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180806 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181009 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181003 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181010 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181127 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181012 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181217 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181220 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180405 F
MouseBrain_HUA_U19_190215 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_170506 F
MouseBrain_HUA_U19_KatieKatie_180405 F
MouseBrain_HUA_U19_190110 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190123 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190117 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190102 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190103 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190108 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190107 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190109 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190118 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190122 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190129 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190206 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190424 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190322 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190422 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190220 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190213 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190219 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190225 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190301 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190306 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190313 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190314 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190319 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190325 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190425 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190315 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190320 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190326 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190426 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190128 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190204 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190207 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190212 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190214 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190226 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190304 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190311 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190318 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190321 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190329 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190430 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180403 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180406 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180410 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180517 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180525 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180605 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180612 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180628 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180702 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180706 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180730 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180802 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180804 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180807 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180820 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180822 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180828 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180829 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180830 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180831 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180905 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180911 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180912 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180913 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_180914 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181112 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181114 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181211 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181101 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181106 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181210 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181002 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181004 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181018 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181025 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181030 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181031 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181105 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_181107 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190724 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190719 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190723 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_170609 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_170702 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_171003 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_171106 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_171107 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_171108 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190308 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190312 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190513 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190514 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190516 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190522 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190523 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190528 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190531 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190604 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190607 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190610 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190611 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190613 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190617 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190618 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190620 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190624 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190625 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190626 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190627 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190628 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190702 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190708 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190710 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190717 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190718 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190720 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP
MouseBrain_HUA_U19_190903 F Region:Brain; Tracer:AAV2/8-Cag-fDIO TVAeGFP